Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: ( A ) Inhibition rate of LOVO cells treated with various concentrations of GA for different times. ( B ) Inhibition rate of LOVO cells treated with the combination of GA and Fe 3 O 4 magnetic nanoparticles for 48 hours. Notes: * P < 0.05 when compared with controls; # P > 0.05 when compared with the group treated with Fe 3 O 4 magnetic nanoparticles. Abbreviations: MNPs-Fe 3 O 4 , magnetic nanoparticles of Fe 3 O 4 ; GA, gambogic acid.

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Inhibition

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Apoptosis ratio of LOVO cells treated with gambogic acid, Fe 3 O 4 magnetic nanoparticles, or magnetic nanoparticles containing Fe 3 O 4 and gambogic acid for 48 hours. ( A ) Controls, ( B ) 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 , ( C ) 0.35 μmol/L gambogic acid, and ( D ) magnetic nanoparticles containing Fe 3 O 4 and gambogic acid (0.35 μmol/L gambogic acid and 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 ).

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Morphological features of LOVO cells after treatment for 48 hours by optical microscope (×400, Wright staining). ( A ) Controls, ( B ) 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 , ( C ) 0.35 μmol/L gambogic acid, and ( D ) magnetic nanoparticles containing Fe 3 O 4 and gambogic acid (0.35 μmol/L gambogic acid with 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 ).

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Microscopy, Wright Stain

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Nucleolus morphological changes of LOVO cells after treatment for 48 hours under fluorescence microscope (×400, DAPI staining). ( A ) Controls, ( B ) 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 , ( C ) 0.35 μmol/L gambogic acid, and ( D ) magnetic nanoparticles containing Fe 3 O 4 and gambogic acid (0.35 μmol/L gambogic acid with 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 ).

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Fluorescence, Microscopy, Staining

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Transcription of PI3K, Akt, Bad, cytochrome C, caspase 9, and caspase 3 gene in LOVO cells after treatment comprising GA with or without MNPs-Fe 3 O 4 for 48 hours. Notes: Lane 1, controls; Lane 2, 60 μg/mL MNPs-Fe 3 O 4 ; Lane 3, 0.35 μmol/L GA; Lane 4, GA-MNPs-Fe 3 O 4 (0.35 μmol/L GA with 60 μg/mL MNPs-Fe 3 O 4 ). * P < 0.05; ** P < 0.01, when compared with control group; # P < 0.05, when compared with the GA group. Abbreviations: MNPs-Fe 3 O 4 , magnetic nanoparticles containing Fe 3 O 4 ; GA, gambogic acid.

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Control

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Expression of PI3K, p-PI3K, Akt, p-Akt, Bad, p-Bad, cytochrome c, pro-caspase 9, caspase 9, and pro-caspase 3, caspase 3 proteins in LOVO cells after treatment comprising GA with or without MNPs-Fe 3 O 4 for 48 hours. Notes: Lane 1, control; lane 2, 60 μg/mL MNPs-Fe 3 O 4 ; lane 3, 0.35 μmol/L GA; lane 4, GA-MNPs-Fe 3 O 4 (0.35 μmol/L GA with 60 μg/mL MNPs-Fe 3 O 4 ). * P < 0.05, ** P < 0.01 compared with the control group; # P < 0.05, when compared with the GA group. Abbreviations: MNPs-Fe 3 O 4 , magnetic nanoparticles containing Fe 3 O 4 ; GA, gambogic acid.

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Expressing, Control

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: The mechanism of GA/GA-MNPs-Fe 3 O 4 on apoptosis of LOVO cells. Notes: →, Promote; ⇢, inhibit; ⇥, block. Abbreviations: MNPs-Fe 3 O 4 , magnetic nanoparticles containing Fe 3 O 4 ; GA, gambogic acid.

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Blocking Assay

Journal: Advanced Science

Article Title: Image‐Guided Magnetic Thermoseed Navigation and Tumor Ablation Using a Magnetic Resonance Imaging System

doi: 10.1002/advs.202105333

Figure Lengend Snippet: Thermal ablation in a mouse subcutaneous tumor model. a) Experimental timeline of BLI, tumor volume measurements, and heating. b) Bioluminescence images of a heated SW1222 Fluc tumor acquired immediately prior to and following heating and a nonheated control tumor, with a thermoseed in place. c) BLI signal integrated over the whole tumor decreased in the treated animals and remained constant for the controls. n = 8 and 7 for the heated and control groups, respectively. d) BLI signal measured longitudinally for treated and control animals, acquired with the thermoseed removed. e) Tumor volume measurements recorded over the course of the experiment. f) Photographs of a treated tumor 3 days prior to and 33 days post treatment show complete ablation of the tumor bulk. g) Hematoxylin and eosin staining of sections of subcutaneous tumors ablated with a 1.5 or 2 mm thermoseed for 1 min or 3 × 1 min show clear regions of necrosis (yellow ROI). The control sample has no distinct area of necrosis but does show the needle insertion tract (blue arrow). Scale bar = 1 mm. In panels (d,e): data shown as mean ± S.D., n = 6 and 7 for heated and control groups, respectively. In panels (c–e): p ‐values are calculated using two‐way repeated measures ANOVA with Bonferroni correction.

Article Snippet: The human colorectal adenocarcinoma cell line SW1222, previously stably transduced with lentiviral vector to express firefly luciferase enzyme (Fluc), were grown in T175 flasks (Fisher Scientific, UK) in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Invitrogen, UK), in a humidified incubator at 37 °C with 95% air and 5% CO 2 .

Techniques: Control, Staining